Of course you can use samples to play the notes in a MIDI, MIDI is just a digital standard for storing a sequence of notes. You can do whatever you want with those.
But now you're grasping at straws, trackers didn't use MIDI, and unlike MIDI, shipped the samples with the tracks, so they'd sound the same wherever they were played.
That there's a superficial similarity is inconsequential, and that you'd bring it up at all, just further crushes your previous claims that trackers were related to earlier 8-bit synth-based music.